Phospho-IKB alpha (S32) Recombinant Rabbit Monoclonal Antibody [ST53-05]

About this product

On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.

Specifications

Catalog number

ET1609-78

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

Abbreviation

IkB-alpha, IkappaBalpha, NFKBIA, IKBA, MAD3, NFKBI

Swiss Prot

P25963 Human, Q9Z1E3 Mouse

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IP

Positive Control

HeLa treated with 20ng/mL TNF-α for 5 minutes cell lysate, NIH/3T3 cells were starved for 18 hours then treated with 20 ng/ml TNF alpha for 5 minutes, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes