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Product details

Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427]

Catalog number ET1609-8

Price for ET1609-8

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Size / count 100 µL

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number
ET1609-8
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
HIST1 cluster, H3J antibody Histone gene cluster 1, H3 histone family, member E antibody Histone gene cluster 1, H3G antibody H3 histone family, member J antibody HIST1 cluster, H3E antibody HIST1 cluster, H3I antibody Histone gene cluster 1, H3C antibody FLJ92264 antibody H3 histone family, member A antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone family, member T antibody H3 histone, family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster, H3A antibody HIST1 cluster, H3B antibody HIST1 cluster, H3C antibody HIST1 cluster, H3D antibody HIST1 cluster, H3F antibody HIST1 cluster, H3G antibody HIST1 cluster, H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 1, H3j antibody histone 3, H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone gene cluster 1, H3 histone family, member A antibody Histone gene cluster 1, H3 histone family, member B antibody Histone gene cluster 1, H3 histone family, member C antibody Histone gene cluster 1, H3 histone family, member D antibody Histone gene cluster 1, H3 histone family, member F antibody Histone gene cluster 1, H3 histone family, member G antibody Histone gene cluster 1, H3 histone family, member H antibody Histone gene cluster 1, H3 histone family, member I antibody Histone gene cluster 1, H3 histone family, member J antibody Histone gene cluster 1, H3A antibody Histone gene cluster 1, H3B antibody Histone gene cluster 1, H3D antibody Histone gene cluster 1, H3E antibody Histone gene cluster 1, H3F antibody Histone gene cluster 1, H3H antibody Histone gene cluster 1, H3I antibody Histone gene cluster 1, H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody H3R2me1 antibody
Abbreviation
Hist1h3a, Hist1h3b,H3R2me1
Swiss Prot
P68431 Human, P84243 Human, Q6NXT2 Human, Q71DI3 Human, Q16695 Human, P84228 Mouse, P68433 Mouse, Q6LED0 Rat
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within human Histone H3 aa 1-49/136.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, ChIP
Positive Control
HepG2, NIH/3T3, PC-3M, human skin tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427]?

The catalog number for Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427] is ET1609-8.

What sizes are available for Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427]?

Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427] is available in the following size(s): 100 µL.

Is Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427] in stock?

Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H3 (mono methyl R2) Recombinant Rabbit Monoclonal Antibody [ST0427] used for?

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form t

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

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