DNA PKcs Recombinant Rabbit Monoclonal Antibody [SC57-08]

About this product

The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V (D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.

Specifications

Catalog number

ET1610-12

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

DNA dependent protein kinase catalytic subunit antibody DNA PK catalytic subunit antibody DNA-dependent protein kinase catalytic subunit antibody DNA-PK catalytic subunit antibody DNA-PKcs antibody DNAPK antibody DNAPK catalytic subunit antibody DNPK 1 antibody DNPK1 antibody Hyper radiosensitivity of murine scid mutation, complementing 1 antibody HYRC 1 antibody HYRC antibody HYRC1 antibody IMD26 antibody p350 antibody p460 antibody PKRDC antibody PRKDC antibody PRKDC_HUMAN antibody Protein Kinase DNA Activated Catalytic Polypeptide antibody XRCC 7 antibody XRCC7 antibody

Abbreviation

DNA-PK catalytic subunit, DNA-PKcs, PRKDC, HYRC, HYRC1

Swiss Prot

P78527 Human, P97313 Mouse,

Cellular Locus

Nucleus, nucleolus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human DNA PKcs aa 4062-4109 / 4128.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, K-562, Hela, MCF-7, human colon carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 469 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes