Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] — image 1 of 3

Product details

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01]

Catalog number ET1610-16

Price for ET1610-16

Size / count 100 µL

About this product

Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Specifications

Catalog number: ET1610-16
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: CYC antibody CYC_HUMAN antibody CYCS antibody Cytochrome c antibody Cytochrome c somatic antibody HCS antibody THC4 antibody
Abbreviation: CYCS
Swiss Prot: P99999 Human, P62897 Mouse, P62898 Rat
Cellular Locus: Mitochondrion intermembrane space.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within human Cytochrome C aa 10-50.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P
Positive Control: HeLa cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, MDA-MB-231 cell lysate, Hela, human liver tissue, mouse liver tissue, rat liver tissue, human kidney tissue, mouse kidney tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000-1:5,000 ;IHC-P: 1:200-1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 12 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01]?

The catalog number for Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] is ET1610-16.

What sizes are available for Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01]?

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] is available in the following size(s): 100 µL.

Is Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] in stock?

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] used for?

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.