UBC9 Recombinant Rabbit Monoclonal Antibody [SC0534]

About this product

UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.

Specifications

Catalog number

ET1610-21

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

C358B7.1 antibody p18 antibody SUMO 1 protein ligase antibody SUMO conjugating enzyme UBC9 antibody SUMO-conjugating enzyme UBC9 antibody SUMO-protein ligase antibody SUMO1 protein ligase antibody UBC9 antibody UBC9_HUMAN antibody UBCE9 antibody Ube2i antibody Ubiquitin carrier protein 9 antibody Ubiquitin carrier protein antibody Ubiquitin carrier protein I antibody Ubiquitin conjugating enzyme 9 antibody Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody

Abbreviation

UBE2I

Swiss Prot

P63279 Human, P63280 Mouse, P63281 Rat

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human UBC9 aa 15-60 / 158.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

HUVEC cell lysate, HepG2 cell lysate, Hela cell lysate, SH-SY5Y cell lysate, A431, HepG2, human kidney tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500- 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

18 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes