Caspase-6 Recombinant Rabbit Monoclonal Antibody [SC56-09]

About this product

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly (ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Specifications

Catalog number

ET1610-24

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Caspase6 Apoptotic protease Mch-2 antibody Apoptotic protease MCH2 antibody CASP-6 antibody CASP6 antibody CASP6_HUMAN antibody Caspase 6 antibody Caspase 6 apoptosis related cysteine protease antibody Caspase 6, apoptosis related cysteine peptidase antibody Caspase-6 subunit p11 antibody Mch2 antibody OTTHUMP00000162742 antibody OTTHUMP00000162743 antibody

Abbreviation

CASP-6, CASP6

Swiss Prot

P55212 Human, O08738 Mouse, O35397 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Caspase-6 aa 210-249 / 293.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

Jurkat cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, C2C12 cell lysate, C6 cell lysate, Rat kidney tissue lysate, Rat colon tissue lysate, Jurkat, human colon tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 33 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes