Phospho-PKA R2 (S99) Recombinant Rabbit Monoclonal Antibody [SC54-04]

About this product

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Specifications

Catalog number

ET1610-29

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

cAMP dependent protein kinase regulatory subunit alpha 2 antibody cAMP dependent protein kinase regulatory subunit RII alpha antibody cAMP dependent protein kinase type II alpha regulatory chain antibody cAMP dependent protein kinase type II alpha regulatory subunit antibody cAMP-dependent protein kinase type II-alpha regulatory subunit antibody KAP2 antibody KAP2_HUMAN antibody MGC3606 antibody PKR 2 antibody PKR2 antibody PRKA R2 antibody PRKAR 2 antibody PRKAR2 antibody PRKAR2A antibody Protein kinase A RII alpha subunit antibody Protein kinase cAMP dependent regulatory type II alpha antibody

Abbreviation

PRKAR2A

Swiss Prot

P13861 Human, P12367 Mouse, P12368 Rat

Cellular Locus

Cell membrane, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Pig

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

Hela, MCF-7, human spleen tissue, mouse testis tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes