Phospho-STAT5 (Y694) Recombinant Rabbit Monoclonal Antibody [SC05-31]

About this product

Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response.

Specifications

Catalog number

ET1610-48

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

MGF antibody Signal transducer and activator of transcription 5A antibody Signal Transducer and Activator of Transcription 5B antibody STA5A_HUMAN antibody STAT 5A antibody STAT 5B antibody STAT5 antibody STAT5A antibody STAT5B antibody Transcription factor STAT5A antibody Transcription factor STAT5B antibody

Abbreviation

STAT5A, STAT5B, STAT5

Swiss Prot

P42229 Human, P51692 Human, P42230 Mouse, P42232 Mouse, P52632 Rat, Q62771 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human STAT5A.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

PC-12 treated with 1mM Sodium orthovanadate for 30 minutes cell lysate, PC-12 cells treated with 1mM Sodium orthovanadate for 30 minutes, Hela cells treated with 100ng/mL IFN alpha for 60 minutes, human breast carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:30,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 91 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes