ATG7 Recombinant Rabbit Monoclonal Antibody [SC06-30]

About this product

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner.

Specifications

Catalog number

ET1610-53

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

1810013K23Rik antibody Apg 7 antibody APG7 autophagy 7 like antibody APG7 autophagy 7-like (S. cerevisiae) antibody APG7 like antibody APG7, S. cerevisiae, homolog of antibody APG7-like antibody APG7L antibody ATG 7 antibody ATG12-activating enzyme E1 ATG7 antibody ATG7 antibody ATG7 autophagy related 7 homolog (S. cerevisiae) antibody ATG7 autophagy related 7 homolog antibody ATG7_HUMAN antibody Atg7l antibody Autophagy 7, S. cerevisiae, homolog of antibody Autophagy related protein 7 antibody Autophagy-related 7 (yeast) antibody Autophagy-related protein 7 antibody DKFZp434N0735 antibody GSA 7 antibody GSA7 antibody hAGP7 antibody Ubiquitin activating enzyme E1 like protein antibody Ubiquitin-activating enzyme E1-like protein antibody Ubiquitin-like modifier-activating enzyme ATG7 antibody

Abbreviation

APG7-like, hAGP7, ATG7

Swiss Prot

O95352 Human, Q641Y5 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

Synthetic peptide within Human ATG7 aa 654-703 / 703.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P, IP, FC

Positive Control

HeLa cell lysate, Jurkat cell lysate, THP-1 cell lysate, human tonsil tissue, human spleen tissue, human kidney tissue, human liver tissue, rat kidney tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 78 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes