PRP19 Recombinant Rabbit Monoclonal Antibody [SC06-53]

About this product

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.

Specifications

Catalog number

ET1610-54

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

hPso4 antibody NMP200 antibody Nuclear matrix protein 200 antibody Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody pre mRNA processing factor 19 antibody Pre-mRNA-processing factor 19 antibody PRP19 antibody PRP19/PSO4 homolog antibody PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody PRP19_HUMAN antibody PRPF19 antibody PSO4 antibody psoralen 4 antibody Senescence evasion factor antibody SNEV antibody UBOX4 antibody

Abbreviation

hPso4, PRPF19

Swiss Prot

Q9UMS4 Human, Q99KP6 Mouse, Q9JMJ4 Rat

Cellular Locus

Nucleus, Cytoplasm, Lipid droplet.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

recombinant protein within Human PRP19 aa 380-524 / 504.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Jurkat, MCF-7, Hela, B16-F1, mouse brain tissue, human lung cancer tissue, mouse kidney tissue, human breast carcinoma tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

55 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes