Topoisomerase I Recombinant Rabbit Monoclonal Antibody [SC69-03]

About this product

DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.

Specifications

Catalog number

ET1610-62

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

DNA topoisomerase 1 antibody DNA topoisomerase I antibody NUP98 fusion gene antibody TOP 1 antibody TOP I antibody TOP1 antibody TOP1_HUMAN antibody TOPI antibody Topoisomerase (DNA) I antibody Topoisomerase 1 antibody Topoisomerase1 antibody TopoisomeraseI antibody Type I DNA topoisomerase antibody

Abbreviation

TOP1

Swiss Prot

P11387 Human, Q04750 Mouse, Q9WUL0 Rat

Cellular Locus

Nucleus, nucleoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human TOP1 aa 1-49 / 765.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

MCF7 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, MCF7, Hela, SHG-44, 293, HepG2, mouse brain tissue, human large intestine tissue, mouse hippocampus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:20,000 ;IF-Cell: 1:50-1:1,000 ;IF-Tissue: 1:50-1:500 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 91 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes