DKK1 Recombinant Rabbit Monoclonal Antibody [SC06-86]

About this product

The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.

Specifications

Catalog number

ET1610-63

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Dickkopf 1 antibody Dickkopf 1 homolog antibody Dickkopf 1 like antibody Dickkopf homolog 1 antibody Dickkopf like protein 1 antibody Dickkopf related protein 1 antibody Dickkopf WNT signaling pathway inhibitor 1 antibody Dickkopf-1 antibody Dickkopf-related protein 1 antibody DKK 1 antibody Dkk-1 antibody Dkk1 antibody DKK1_HUMAN antibody hDkk 1 antibody hDkk-1 antibody SK antibody

Abbreviation

Dickkopf-1, Dkk-1, hDkk-1, DKK1

Swiss Prot

O94907 Human

Cellular Locus

Secreted.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

Synthetic peptide within Human DKK1 aa 225-266 / 266.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Caco-2 cell lysates, Hela, HepG2, NCCIT, human uterus tissue, human liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50-1:500 ;IF-Tissue: 1:50-1:500 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes