PKR Recombinant Rabbit Monoclonal Antibody [SC06-37]

About this product

An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation.

Specifications

Catalog number

ET1610-84

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Double stranded RNA activated protein kinase; antibody E2AK2_HUMAN antibody eIF-2A protein kinase 2 antibody EIF2AK1 antibody EIF2AK2 antibody Eukaryotic translation initiation factor 2 alpha kinase 2 antibody Eukaryotic translation initiation factor 2-alpha kinase 2 antibody HGNC:9437 antibody Interferon induced double stranded RNA activated protein kinase antibody Interferon inducible elF2 alpha kinase antibody Interferon inducible RNA dependent protein kinase antibody Interferon-induced, double-stranded RNA-activated protein kinase antibody Interferon-inducible RNA-dependent protein kinase antibody MGC126524 antibody P1/eIF-2A protein kinase antibody P1/eIF2A protein kinase antibody p68 kinase antibody PKR antibody PPP1R83 antibody PRKR antibody Protein kinase interferon inducible double stranded RNA dependent antibody Protein kinase RNA activated antibody Protein kinase RNA-activated antibody Protein phosphatase 1 regulatory subunit 83 antibody Serine/threonine protein kinase TIK antibody Tyrosine protein kinase EIF2AK2 antibody

Abbreviation

eIF-2A protein kinase 2, PKR, Protein kinase R, EIF2AK2

Swiss Prot

P19525 Human

Cellular Locus

Nucleus, Cytoplasm, perinuclear region.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human PKR aa 500-540.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

MCF-7 cell lysate, Hela cell lysate, MCF-7, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:100;IF-Tissue: 1:50-1:100;IHC-P: 1:50-1:100;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 62 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes