GAP43 Recombinant Rabbit Monoclonal Antibody [SC60-06]

About this product

GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. GAP43, the consensus choice for its designation, is a nervous system-specific protein that is attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4, though it can exist in the non-bound form in the cytoplasm. This dual sequence enables the association of phosphatidylinositol-4,5-bisphosphate [PI (4,5)P2] or PIP2, with actin, facilitating the latter's polymerization thereby regulating neuronal structure. GAP-43 is also a protein kinase C (PKC) substrate. Phosphorylation of serine-41 on GAP-43 by PKC regulates neurite formation, regeneration, and synaptic plasticity.

Specifications

Catalog number

ET1610-94

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Axonal membrane protein GAP 43 antibody Axonal membrane protein GAP-43 antibody B 50 antibody Calmodulin binding protein P 57 antibody F1 antibody GAP 43 antibody GAP43 antibody Growth Associated Protein 43 antibody Growth-associated protein 43 antibody Nerve Growth Related Peptide antibody Nerve growth related peptide GAP43 antibody NEUM_HUMAN antibody Neural phosphoprotein B 50 antibody Neural phosphoprotein B-50 antibody Neuromodulin antibody Neuron growth associated protein 43 antibody PP46 antibody Protein F1 antibody QtrA-11580 antibody QtrA-13071 antibody

Abbreviation

GAP43

Swiss Prot

P17677 Human, P06837 Mouse, P07936 Rat

Cellular Locus

Cell membrane, growth cone membrane, filopodium membrane, Cytoplasm, synapse, perikaryon, dendrite, axon.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human GAP43 aa 189-238 / 238.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human glioma tissue, Neuro-2a, rat hippocampus tissue lysate, mouse hippocampus tissue lysate, mouse brain tissue, rat brain tissue, rat hippocampus tissue, SH-SY5Y.

Concentration

1 mg/mL

Dilution

WB: 1:5,000-1:100,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:10,000-1:15,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 25 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes