Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05]

Catalog number ET1610-95

Price for ET1610-95

Size / count 100 µL

About this product

Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.

Specifications

Catalog number: ET1610-95
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Caspase9 APAF-3 antibody APAF3 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase 9 Dominant Negative antibody Caspase 9c antibody Caspase-9 antibody Caspase-9 subunit p10 antibody ICE LAP6 antibody ICE like apoptotic protease 6 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody MCH6 antibody PPP1R56 antibody protein phosphatase 1, regulatory subunit 56 antibody RNCASP9 antibody
Abbreviation: CASP-9, APAF-3, ICE-LAP6, CASP9
Swiss Prot: P55211 Human, Q8C3Q9 Mouse
Cellular Locus: Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.
Host: Rabbit
Species Reactivity: Human, Mouse
Immunogen: Recombinant protein within Human Caspase-9 aa 95-335 / 416.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IP
Positive Control: HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, HeLa treated with 3μM staurosporine for 4 hours cell lysate, C2C12 cell lysate, Jurkat cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 46 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05]?

The catalog number for Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] is ET1610-95.

What sizes are available for Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05]?

Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] is available in the following size(s): 100 µL.

Is Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] in stock?

Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05] used for?

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.