Hes1 Recombinant Rabbit Monoclonal Antibody [SC06-21]

About this product

Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila. HES1 is one of the seven members of the Hes gene family (HES1-7). Hes genes code nuclear proteins that suppress transcription. This protein belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box promoter region rather than the canonical enhancer box (E-box). As a member of the bHLH family, it is a transcriptional repressor that influences cell proliferation and differentiation in embryogenesis. HES1 regulates its own expression via a negative feedback loop, and oscillates with approximately 2-hour periodicity.

Specifications

Catalog number

ET1610-97

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

bHLHb39 antibody C-HAIRY1 antibody c-hairy1A antibody Class B basic helix-loop-helix protein 39 antibody FLJ20408 antibody Hairy and enhancer of split 1 (Drosophila) antibody Hairy and enhancer of split 1 antibody Hairy homolog (Drosophila) antibody Hairy homolog antibody Hairy like antibody Hairy, Drosophila, homolog of antibody Hairy-like protein antibody Hairy/enhancer of split, Drosophila, homolog of, 1 antibody HAIRY1 antibody HES-1 antibody hes1 antibody Hes1 hairy and enhancer of split 1 (Drosophila) antibody HES1_HUMAN antibody HHL antibody HL antibody HRY antibody MGC129109 antibody OTTHUMP00000209031 antibody RHL antibody Transcription factor HES-1 antibody

Abbreviation

bHLHb39, hHL, HES1

Swiss Prot

Q14469 Human, P35428 Mouse, Q04666 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Hes1 aa 298-241 / 280.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IHC-Fr

Positive Control

SH-SY5Y cell lysate, MDA-MB-231 cell lysate, MCF7 cell lysate, SK-MEL-28 cell lysate, SH-SY5Y, mouse brain tissue, rat brain tissue, human breast carcinoma tissue, mouse cerebellum tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:2,000 ;IF-Tissue: 1:200 ;IHC-P: 1:50-1:400 ;FC: 1:1:1,000 ;IHC-Fr: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 30 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes