RhoA Recombinant Rabbit Monoclonal Antibody [SN0612]

About this product

Transforming protein RhoA, also known as Ras homolog family member A (RhoA), is a small GTPase protein in the Rho family of GTPases that in humans is encoded by the RHOA gene. While the effects of RhoA activity are not all well known, it is primarily associated with cytoskeleton regulation, mostly actin stress fibers formation and actomyosin contractility. It acts upon several effectors. Among them, ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (Diaphanous Homologue 1, a.k.a. hDia1, homologue to mDia1 in mouse, diaphanous in Drosophila) are the best described. RhoA, and the other Rho GTPases, are part of a larger family of related proteins known as the Ras superfamily, a family of proteins involved in the regulation and timing of cell division. RhoA is one of the oldest Rho GTPases, with homologues present in the genomes since 1.5 billion years. As a consequence, RhoA is somehow involved in many cellular processes which emerged throughout evolution. RhoA specifically is regarded as a prominent regulatory factor in other functions such as the regulation of cytoskeletal dynamics, transcription, cell cycle progression and cell transformation.

Specifications

Catalog number

ET1611-10

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Aplysia ras related homolog 12 antibody ARH12 antibody ARHA antibody H 12 antibody H12 antibody Oncogene RHO H12 antibody Ras homolog family member A antibody Ras homolog gene family member A antibody Rho A antibody Rho cDNA clone 12 antibody RHO H12 antibody RHO12 antibody RHOA antibody RHOA_HUMAN antibody RHOH12 antibody Small GTP binding protein Rho A antibody Transforming protein Rho A antibody Transforming protein RhoA antibody

Abbreviation

h12, RHOA

Swiss Prot

P61586 Human, Q9QUI0 Mouse, P61589 Rat

Cellular Locus

Cell membrane, Cytoplasm, Cleavage furrow, Midbody, Cell projection.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human aa 144-193 / 193.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, FC, IHC-P, IF-Cell

Positive Control

HUVEC cell lysate, THP-1 cell lysate, HeLa cell lysate, SiHa cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue lysate, PC-12, human liver cancer tissue, mouse brain tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Tissue: 1:50-1:200 ;FC: 1:50-1:100 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes