Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36]

Catalog number ET1611-18

Price for ET1611-18

Size / count 100 µL

About this product

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

Specifications

Catalog number: ET1611-18
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: 60S acidic ribosomal protein P1 antibody AA409079 antibody AI325195 antibody AU020965 antibody ik:tdsubc_2g1 antibody M (2)21C antibody MGC137236 antibody OTTHUMP00000004008 antibody p32 antibody p34 antibody RCJMB04_6d17 replication protein A2, 32kDa antibody REPA2 antibody Replication factor A protein 2 antibody Replication protein A 32 kDa subunit antibody Replication protein A 32kDa subunit antibody Replication protein A 34 kDa subunit antibody Replication protein A antibody Replication Protein A2 (32kDa) antibody Replication protein A2 antibody Replication protein A2, 32kDa antibody RF-A protein 2 antibody Rf-A2 antibody RFA antibody RFA2_HUMAN antibody RP-A p32 antibody RP-A p34 antibody RP21C antibody RPA 2 antibody RPA 32 antibody RPA antibody Rpa2 antibody RPA32 antibody RPA34 antibody RpLP1 antibody RpP2 antibody xx:tdsubc_2g1 antibody zgc:109822 antibody
Abbreviation: RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34
Swiss Prot: P15927 Human, Q62193 Mouse, Q63528 Rat
Cellular Locus: Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr21 of Human RPA32 / RPA2 aa 1-50 / 270.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IP, IF-Cell
Positive Control: HeLa whole cell lysate, HeLa treated with 1uM Camptothecin for 1 hour whole cell lysate, HEK-293 whole cell lysate, HEK-293 treated with UV for 20 minutes then recover 2 hours whole cell lysate, HEK-293 treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa, Hela cell lysate, HL-60 cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:50
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 29 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36]?

The catalog number for Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36] is ET1611-18.

What sizes are available for Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36]?

Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36] is available in the following size(s): 100 µL.

Is Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36] in stock?

Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36] used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.