Phospho-STAT1 (S727) Recombinant Rabbit Monoclonal Antibody [SN67-04]

About this product

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Specifications

Catalog number

ET1611-20

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Signal transducer and activator of transcription 1 91kD antibody CANDF7 antibody DKFZp686B04100 antibody IMD31A antibody IMD31B antibody IMD31C antibody ISGF 3 antibody ISGF-3 antibody OTTHUMP00000163552 antibody OTTHUMP00000165046 antibody OTTHUMP00000165047 antibody OTTHUMP00000205845 antibody Signal transducer and activator of transcription 1 91kDa antibody Signal transducer and activator of transcription 1 antibody Signal transducer and activator of transcription 1, 91kD antibody Signal transducer and activator of transcription 1-alpha/beta antibody STAT 1 antibody Stat1 antibody STAT1_HUMAN antibody STAT91 antibody Transcription factor ISGF 3 components p91 p84 antibody Transcription factor ISGF-3 components p91/p84 antibody

Abbreviation

STAT1

Swiss Prot

P42224 Human, P42225 Mouse,

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser727 of Human STAT1 aa 701-750 / 750.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

HeLa cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa, human breast invasive ductal tumor tissue, rat colon tissue, mouse colon tissue, SiHa cell lysates, human liver carcinoma tissue, human lung carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:5,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:2,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 87 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes