Nestin Recombinant Rabbit Monoclonal Antibody [SN06-27]

About this product

Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

Specifications

Catalog number

ET1611-21

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ESTM 46 antibody FLJ 21841 antibody FLJ21841 antibody Intermediate filament protein antibody Nbla00170 antibody nes antibody NEST_HUMAN antibody Nestin antibody

Abbreviation

NES

Swiss Prot

P48681 Human

Cellular Locus

Cytoplasm, Intermediate filament.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Nestin aa 531-580 / 1,621.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IF-Cell, IF-Tissue, IHC-P, WB

Positive Control

HeLa cell lysates, human kidney tissue, human colon carcinoma tissue, U-87 MG.

Concentration

1 mg/mL

Dilution

IF-Cell: 1:100 ;IF-Tissue: 1:200 ;IHC-P: 1:1,000 ;WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 177 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes