Phospho-PDGFR beta (Y740) Recombinant Rabbit Monoclonal Antibody [SN0646]

About this product

Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor. Mutations in PDGFRB are mainly associated with the clonal eosinophilia class of malignancies.

Specifications

Catalog number

ET1611-29

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Beta platelet derived growth factor receptor antibody Beta-type platelet-derived growth factor receptor antibody CD 140B antibody CD140 antigen-like family member B antibody CD140b antibody CD140b antigen antibody IBGC4 antibody IMF1 antibody JTK12 antibody OTTHUMP00000160528 antibody PDGF R beta antibody PDGF-R-beta antibody PDGFR 1 antibody PDGFR antibody PDGFR beta antibody PDGFR1 antibody PDGFRB antibody PGFRB_HUMAN antibody Platelet derived growth factor receptor 1 antibody Platelet derived growth factor receptor beta antibody Platelet derived growth factor receptor beta polypeptide antibody

Abbreviation

PDGF-R-beta, PDGFR-beta, PDGFR-1, PDGFRB, Pdgfrb

Swiss Prot

P09619 Human, P05622 Mouse, Q05030 Rat

Cellular Locus

Cell membrane, Cytoplasmic vesicle.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGFR beta.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue

Positive Control

NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate, C2C12, Hela, L6.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 124 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes