Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03]

About this product

Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLCγ is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.

Specifications

Catalog number

ET1611-41

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AGTR 2 antibody Agtr2 antibody AGTR2_HUMAN antibody angiotensin II receptor type 2 antibody Angiotensin II type-2 receptor antibody Angiotensin receptor 2 antibody AT 2 antibody AT2 antibody ATGR 2 antibody ATGR2 antibody MRX 88 antibody MRX88 antibody Type 2 angiotensin II receptor antibody Type-2 angiotensin II receptor antibody

Abbreviation

AT2, AGTR2

Swiss Prot

P50052 Human, P35374 Mouse, P35351 Rat

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Angiotensin II Type 2 Receptoraa 311-363 / 363.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP

Positive Control

293T cell lysate, Hela cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes