Glutaminase Recombinant Rabbit Monoclonal Antibody [SN68-09]

About this product

Glutamine is an important molecule involved in several cellular functions, including nitrogen and carbon transport, hepatic urea synthesis, renal ammoniagenesis, and gluconeogenesis. Glutamine is catabolized by either the liver-type (LGA) or kidney-type (KGA) glutaminase. KGA is mitochondrial specific protein whose expression in kidney is increased during metabolic acidosis. This process is mediated by an 8-base AU-sequence in KGA that functions as a pH-response element. The human KGA gene maps to chromosome 2, and produces three isoforms, designated KGA, GAC, and GAM, by alternative splicing. KGA is synthesized as a cytosolic protein that is transported to the mitochondria as an intermediate protein, and is further cleaved into the KGA isoform and the GAC isoform. The processing of the GAM isoform is unclear. The KGA isoform is abundant in brain and kidney, while the GAC isoform is principally expressed in cardiac muscle and pancreas. The GAM isoform is solely expressed in cardiac and skeletal muscle.

Specifications

Catalog number

ET1611-5

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AAD20 antibody DKFZp686O15119 antibody FLJ10358 antibody GAC antibody GAM antibody GLS antibody GLS1 antibody GLSK_HUMAN antibody Glutaminase C antibody Glutaminase kidney isoform antibody Glutaminase phosphate activated antibody K-glutaminase antibody KGA antibody KIAA0838 antibody L-glutamine amidohydrolase antibody mitochondrial antibody

Abbreviation

GLS

Swiss Prot

O94925 Human, D3Z7P3 Mouse, P13264 Rat

Cellular Locus

Cytoplasm, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide within human Glutaminase aa 50-100.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

293T cell lysate, HeLa cell lysate, K-562 cell lysate, PC-12 cell lysate, Rat brain tissue lysate, HeLa, human kidney tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 74 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes