PP2A alpha + beta Recombinant Rabbit Monoclonal Antibody [SN70-08]

About this product

The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.

Specifications

Catalog number

ET1611-54

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

PP2A A antibody PP2A alpha antibody PP2A B antibody PP2A beta antibody PP2A-alpha antibody PP2AA_HUMAN antibody PP2Ac antibody PP2CB antibody PPP2CA antibody PPP2CB antibody Protein phosphatase 2 catalytic subunit alpha isoform antibody Protein phosphatase 2 catalytic subunit beta isoform antibody Replication protein C antibody RP C antibody RP-C antibody Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

Abbreviation

PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB

Swiss Prot

P62714 Human, P67775 Human, P62715 Mouse, P63330 Mouse, P62716 Rat, P63331 Rat

Cellular Locus

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human PP2A alpha aa 260-309 / 309.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IP

Positive Control

A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, PANC-1, Hela, 293, L6, rat kidney tissue, human tonsil tissue, human pancreas tissue, mouse brain tissue, mouse stomach tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:100-1:200;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes