SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09]

About this product

SOX-9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene. SOX-9 also plays a pivotal role in male sexual development; by working with Sf1, SOX-9 can produce AMH in Sertoli cells to inhibit the creation of a female reproductive system. It also interacts with a few other genes to promote the development of male sexual organs. The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene. Next, Sox9 activates FGF9 and forms feedforward loops with FGF9[ and PGD2. These loops are important for producing SOX-9; without these loops, SOX-9 would run out and the development of a female would almost certainly ensue. Activation of FGF9 by SOX-9 starts vital processes in male development, such as the creation of testis cords and the multiplication of Sertoli cells.

Specifications

Catalog number

ET1611-56

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

campomelic dysplasia autosomal sex reversal antibody CMD 1 antibody CMD1 antibody CMPD 1 antibody CMPD1 antibody SOX 9 antibody Sox9 antibody SOX9_HUMAN antibody SRA 1 antibody SRA1 antibody SRXX2 antibody SRXY10 antibody SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody SRY (sex determining region Y) box 9 antibody SRY (sex determining region Y)-box 9 antibody SRY (sex-determining region Y)-box 9 protein antibody SRY related HMG box gene 9 antibody Transcription factor SOX 9 antibody Transcription factor SOX-9 antibody transcription factor SOX9 antibody

Abbreviation

SOX9

Swiss Prot

P48436 Human, Q04887 Mouse, F1LYL9 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human SOX9 aa 140-340.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IP, FC, CUT&Tag-seq, mIHC

Positive Control

SW480 cell lysate, MDA-MB-231 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, MDA-MB-231, human tonsil tissue, human breast carcinoma tissue, mouse liver tissue, human colon tissue, human thyroid tissue, human breast tissue, human small intestine tissue, human prostate carcinoma tissue, human stomach carcinoma tissue, human lung carcinoma tissue, rat colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:10,000 ;IF-Cell: 1:500 ;IHC-P: 1:50-1:1,000 ;FC: 1:2,000 ;IP: Use at an assay dependent concentration. ;mIHC: 1:1,500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 56 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes