IRF3 Recombinant Rabbit Monoclonal Antibody [SD2062]

About this product

Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP).

Specifications

Catalog number

ET1612-14

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

IIAE7 antibody Interferon regulatory factor 3 antibody IRF 3 antibody IRF-3 antibody IRF3 antibody IRF3_HUMAN antibody MGC94729 antibody

Abbreviation

IRF-3

Swiss Prot

Q14653 Human, P70671 Mouse

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human IRF3 aa 71-120 / 427.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, A549 cell lysate, THP-1 cell lysate, Hela-si NT cell lysate, Hela-si IRF3 cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, mouse liver tissue lysate, Hela, MCF-7, PANC-1, human breast carcinoma tissue, human kidney tissue, human pancreas tissue, human tonsil tissue, mouse colon tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat colon tissue, rat heart tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:2,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 47 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes