Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] — image 1 of 3

Product details

Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63]

Catalog number ET1612-25

Price for ET1612-25

Size / count 100 µL

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number: ET1612-25
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: H2B GL105 antibody H2B histone family member O antibody H2B histone family member S antibody H2B.1 antibody H2B.1 B antibody H2B.b antibody H2B.c antibody H2B.d antibody H2B.e antibody H2B.f antibody H2B.j antibody H2B.q antibody H2B/b antibody H2B/c antibody H2B/d antibody H2B/e antibody H2B/f antibody H2B/j antibody H2B/o antibody H2B/q antibody H2BFB antibody H2BFC antibody H2BFD antibody H2BFE antibody H2BFF antibody H2BFJ antibody H2BFO antibody H2BFQ antibody H2BFS antibody HIRIP2 antibody HIST1H2BB antibody HIST1H2BD antibody HIST1H2BH antibody HIST1H2BL antibody HIST1H2BM antibody HIST1H2BN antibody HIST2H2BE antibody Histone H2B antibody Histone H2B type 1 B antibody Histone H2B type 1 D antibody Histone H2B type 1 H antibody Histone H2B type 1 L antibody Histone H2B type 1 M antibody Histone H2B type 1 N antibody Histone H2B type 2 E antibody histone protein antibody
Abbreviation: Hist1h2ba
Swiss Prot: O60814 Human, P10854 Mouse, Q64475 Mouse, Q00729 Rat
Cellular Locus: Nucleus, Chromosome.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within human Histone H2B aa 110 to the C-terminus.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, ChIP
Positive Control: Hela cell lysate, PC-12 cell lysate, A431, human tonsil tissue, human liver tissue, mouse liver tissue, mouse testis tissue, mouse colon tissue, human breast carcinoma tissue, Hela.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 14 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63]?

The catalog number for Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] is ET1612-25.

What sizes are available for Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63]?

Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] is available in the following size(s): 100 µL.

Is Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] in stock?

Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H2B Recombinant Rabbit Monoclonal Antibody [SD20-63] used for?

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.