Phospho-Cyclin E1 (T77) Recombinant Rabbit Monoclonal Antibody [SD2025]

About this product

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Specifications

Catalog number

ET1612-31

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CCNE antibody Ccne1 antibody CCNE1_HUMAN antibody cyclin E variant ex5del antibody cyclin E variant ex7del antibody Cyclin E1 antibody Cyclin Es antibody Cyclin Et antibody CyclinE antibody G1/S specific cyclin E antibody G1/S-specific cyclin-E1 antibody

Abbreviation

CCNE1

Swiss Prot

P24864 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr77 of Human Cyclin E1 aa 51-100 / 410.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

SW480, Hela, HepG2, human colon carcinoma tissue, human testis tissue, mouse testis tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 47 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes