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Product details

Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1]

Catalog number ET1612-32

Price for ET1612-32

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Size / count 100 µL

About this product

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Specifications

Catalog number
ET1612-32
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
Drosophila, homolog of, MADR2 antibody hMAD-2 antibody HsMAD2 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mother against DPP homolog 2 antibody Mothers against decapentaplegic homolog 2 antibody Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody Mothers against DPP homolog 2 antibody OTTHUMP00000163489 antibody Sma and Mad related protein 2 antibody Sma- and Mad-related protein 2 MAD antibody SMAD 2 antibody SMAD family member 2 antibody SMAD, mothers against DPP homolog 2 antibody SMAD2 antibody SMAD2_HUMAN antibody
Abbreviation
MAD homolog 2, Mothers against DPP homolog 2, hMAD-2, SMAD 2, Smad2, hSMAD2
Swiss Prot
Q15796 Human, Q62432 Mouse, O70436 Rat
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser250 of Human Smad2 aa 221-270 / 467.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, FC, IP
Positive Control
HeLa cell lysate, HeLa treated with 200nM PMA for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;FC: 1:50-1:100 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 52 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1]?

The catalog number for Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] is ET1612-32.

What sizes are available for Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1]?

Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] is available in the following size(s): 100 µL.

Is Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] in stock?

Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] used for?

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1)

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

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