RUNX1+RUNX2+RUNX3 Recombinant Rabbit Monoclonal Antibody [SD0803]

About this product

The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 promotes axonal growth, is selectively expressed in neural crest-derived TrkA+ sensory neurons and mediates TrkA transactivation in migratory neural crest cells. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the diagnosis and risk assessment of gastric cancer.

Specifications

Catalog number

ET1612-49

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Runt-related transcription factor 1 Acute myeloid leukemia 1 protein Core-binding factor subunit alpha-2 CBF-alpha-2 Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit PEA2-alpha B PEBP2-alpha B SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit RUNX1 AML1 CBFA2 Runt-related transcription factor 2 Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 CBF-alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 OSF-2 Polyomavirus enhancer-binding protein 2 alpha A subunit PEA2-alpha A PEBP2-alpha A SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit RUNX2 AML3 CBFA1 OSF2 PEBP2A Runt-related transcription factor 3 Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 CBF-alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit PEA2-alpha C PEBP2-alpha C SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core-binding factor alpha C subunit RUNX3 AML2 CBFA3 PEBP2A3

Abbreviation

CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B, RUNX1, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2, CBF-alpha-3, PEA2-alpha C, PEBP2-alpha C, RUNX3

Swiss Prot

Q01196 Human, Q13761 Human, Q13950 Human, Q03347 Mouse, Q08775 Mouse, Q64131 Mouse, Q63046 Rat, Q9Z2J9 Rat

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human RUNX1 aa 404-453 / 453.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

Saos-2 cell lysate, NIH/3T3 cell lysate, SHG-44 cell lysate, Mouse thymus tissue lysate, SHG-44, mouse testis tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

49 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes