AMPK beta 1 Recombinant Rabbit Monoclonal Antibody [SD082-06]

About this product

AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

Specifications

Catalog number

ET1612-56

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

1300015D22Rik antibody 5'-AMP-activated protein kinase subunit beta-1 antibody 5'-AMP-activated protein kinase beta-1 subunit antibody AAKB1_HUMAN antibody AMP-activated protein kinase beta subunit antibody AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody AMP-activated, noncatalytic, beta-1 antibody AMPK antibody AMPK beta 1 chain antibody AMPK subunit beta-1 antibody AMPK-BETA-1 antibody AMPKb antibody AU021155 antibody E430008F22 antibody HAMPKb antibody MGC17785 antibody PRKAB1 antibody Protein kinase AMP activated non catalytic subunit beta 1 antibody protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody protein kinase, AMP-activated, noncatalytic, beta-1 antibody

Abbreviation

AMPK subunit beta-1, AMPKb, PRKAB1

Swiss Prot

Q9Y478 Human, Q9R078 Mouse, P80386 Rat

Cellular Locus

Cytosol, nucleoplasm, nucleus, cytoplasm, nucleotide-activated protein kinase complex.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human AMPK beta 1 aa 90-130.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

HeLa cell lysate, A431 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, human smooth muscle tissue, A431, C2C12.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 30 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes