DYNLL1 Recombinant Rabbit Monoclonal Antibody [SD08-04]

About this product

Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. The highly conserved DYNLL proteins were originally identified as light chains for microtubule-based motor protein Dynein. In mammals there are two closely related isoforms expressed, DYNLL1 and DYNLL2 which share 93% sequence identity at the protein level. DYNLL1 (Dynein light chain 1) also designated, DLC8 or PIN (Protein inhibitor of neuronal nitric oxide synthase) has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition. Dimerization is required for NOS1 activity and DYNLL1 has been shown to destabilize the NOS1 dimer. Nitric oxide may be involved in several processes such as apoptosis, synaptogenesis and neuronal development; thus DYNLL1 is implicated in these processes as well. DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain.

Specifications

Catalog number

ET1612-64

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

8 kDa dynein light chain antibody 8kDLC antibody Cytoplasmic dynein light polypeptide antibody DLC1 antibody DLC8 antibody DNCL1 antibody DNCLC1 antibody DYL1_HUMAN antibody Dynein , cytoplasmic, light chain 1 antibody Dynein light chain 1 cytoplasmic antibody Dynein light chain 1, cytoplasmic antibody Dynein light chain LC8 type 1 antibody Dynein light chain LC8-type 1 antibody Dynein, cytoplasmic, light polypeptide 1 antibody Dynein, light chain, LC8-type 1 antibody DYNLL1 antibody HDLC1 antibody LC8 antibody LC8a antibody MGC126137 antibody MGC126138 antibody MGC72986 antibody PIN antibody Protein inhibitor of neuronal nitric oxide synthase antibody Protein inhibitor of neuronal NOS antibody

Abbreviation

DLC8, PIN, DYNLL1

Swiss Prot

P63167 Human, P63168 Mouse, P63170 Rat

Cellular Locus

Mitochondrion, Nucleus, centrosome, cytoskeleton.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human DYNLL1 aa 1-50 / 89.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate, MCF7, C2C12, L6, mouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 10 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes