Cdk9 Recombinant Rabbit Monoclonal Antibody [SD204-07]

About this product

A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1–9, PISSLRE, KKIALRE, PITSLRE, and PCTAIRE 1–3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1–3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.

Specifications

Catalog number

ET1612-78

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

C-2K antibody CDC2 related kinase antibody CDC2L4 antibody Cdk 9 antibody Cdk9 antibody CDK9_HUMAN antibody Cell division cycle 2-like protein kinase 4 antibody Cell division protein kinase 9 antibody CTK1 antibody Cyclin dependent kinase 9 antibody Cyclin-dependent kinase 9 antibody PITALRE antibody Serine/threonine-protein kinase PITALRE antibody TAK antibody Tat associated kinase complex catalytic subunit antibody

Abbreviation

CDK9

Swiss Prot

P50750 Human, Q99J95 Mouse, Q641Z4 Rat

Cellular Locus

Nucleus, PML body, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Cdk9 aa 323-372 / 372.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

HeLa cell lysate, HT-29 cell lysate, RAW264.7 cell lysate, F9 cell lysate, PC-12 cell lysate, C6 cell lysate, Hela, A549, SW480, human lung carcinoma tissue, human breast carcinoma tissue, human tonsil tissue, human colon carcinoma tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes