UBA1 Recombinant Rabbit Monoclonal Antibody [SD08-62]

About this product

Ubiquitin-like modifier activating enzyme 1 (UBA1) is an enzyme which in humans is encoded by the UBA1 gene. UBA1 participates in ubiquitination and the NEDD8 pathway for protein folding and degradation, among many other biological processes. This protein has been linked to X-linked spinal muscular atrophy type 2, neurodegenerative diseases, and cancers. The protein encoded by this gene catalyzes the first step in ubiquitin conjugation, or ubiquitination, to mark cellular proteins for degradation. Specifically, UBA1 catalyzes the ATP-dependent adenylation of ubiquitin, thereby forming a thioester bond between the two. It also continues to participate in subsequent steps of ubiquination as a Ub carrier. There are only two human ubiquitin-activating enzymes, UBA1 and UBA6, and thus UBA1 is largely responsible for protein ubiquitination in humans. Through its central role in ubiquitination, UBA1 has been linked to cell cycle regulation, endocytosis, signal transduction, apoptosis, DNA damage repair, and transcriptional regulation. Additionally, UBA1 helps regulate the NEDD8 pathway, thus implicating it in protein folding, as well as mitigating the depletion of ubiquitin levels during stress.

Specifications

Catalog number

ET1612-82

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A1S9 antibody A1S9 protein antibody A1S9T and BN75 temperature sensitivity complementing antibody A1S9T antibody A1ST antibody AMCX1 antibody CFAP124 antibody CTD-2522E6.1 antibody GXP 1 antibody GXP1 antibody MGC4781 antibody POC20 antibody POC20 centriolar protein homolog antibody Protein A1S9 antibody SMAX2 antibody Uba1 antibody UBA1, ubiquitin-activating enzyme E1 homolog A antibody UBA1_HUMAN antibody UBA1A antibody UBE 1 antibody UBE 1X antibody UBE1 antibody UBE1X antibody Ubiquitin activating enzyme E1 antibody Ubiquitin-activating enzyme E1 antibody Ubiquitin-like modifier-activating enzyme 1 antibody

Abbreviation

UBA1

Swiss Prot

P22314 Human, Q02053 Mouse, Q5U300 Rat

Cellular Locus

Nucleus, Mitochondrion, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human UBA1 aa 1,009-1,058 / 1,058.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

K-562 cell lysate, HeLa cell lysate, U-87 MG cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, HeLa, NIH/3T3, PC-12, human colon cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 118 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes