Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]

About this product

Collagen VI (ColVI) is a type of collagen primarily associated with the extracellular matrix of skeletal muscle. ColVI maintains regularity in muscle function and stabilizes the cell membrane. It is synthesized by a complex, multistep pathway that leads to the formation of a unique network of linked microfilaments located in the extracellular matrix (ECM). ColVI plays a vital role in numerous cell types, including chondrocytes, neurons, myocytes, fibroblasts, and cardiomyocytes. ColVI molecules are made up of three alpha chains: α1 (VI), α2 (VI), and α3 (VI). It is encoded by 6 genes: COL6A1, COL6A2, COL6A3, COL6A4, COL6A5, and COL6A6. The chain lengths of α1 (VI) and α2 (VI) are about 1,000 amino acids. The chain length of α3 (VI) is roughly a third larger than those of α1 (VI) and α2 (VI), and it consists of several spliced variants within the range of 2,500 to 3,100 amino acids. The first two alpha chains subunits of ColVI have a molecular weight of 140-150 KDa and the third polypeptide chain is larger with a molecular weight of 250-300kDa. ColVI is also found in the skin, lungs, blood vessels, cornea and intervertebral disc. It also forms part of the peripheral nerves, brain, myocardium and adipose tissue.

Specifications

Catalog number

ET1612-91

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Alpha 1 (VI) chain (61 AA) antibody CO6A1_HUMAN antibody COL6A1 antibody Collagen alpha-1 (VI) chain antibody Collagen type VI alpha 1 antibody Collagen VI alpha 1 polypeptide antibody CollagenVI antibody Human mRNA for collagen VI alpha 2 C terminal globular domain antibody OPLL antibody PP3610 antibody

Abbreviation

COL6A1, COL6A2

Swiss Prot

P12109 Human, P12110 Human, P12111 Human, Q02788 Mouse, Q04857 Mouse

Cellular Locus

Extracellular matrix.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Collagen VI alpha1 aa 17-255 / 1,028.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

NIH/3T3 cell lysate, human kidney tissue lysate, rat heart tissue lysate, rat kidney tissue lysate, Hela, A549, HepG2, RH-35, human lung tissue, human liver tissue, mouse colon tissue, human colon tissue, human skin tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 109 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes