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Product details

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]

Catalog number ET1612-94

Price for ET1612-94

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Size / count 100 µL

About this product

Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Specifications

Catalog number
ET1612-94
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
ANAPC3 antibody Anaphase Promoting Complex 3 antibody Anaphase promoting complex protein 3 antibody Anaphase Promoting Complex Subunit 3 antibody Anaphase-promoting complex subunit 3 antibody APC 3 antibody APC3 antibody Cdc 27 antibody Cdc27 antibody CDC27 homolog antibody CDC27_HUMAN antibody CDC27Hs antibody Cell Division Cycle 27 antibody Cell division cycle protein 27 homolog antibody D0S1430E antibody D17S978E antibody H NUC antibody H-NUC antibody HNUC antibody Nuc2 homolog antibody
Abbreviation
APC3, CDC27Hs, CDC27
Swiss Prot
P30260 Human, A2A6Q5 Mouse, Q4V8A2 Rat
Cellular Locus
Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein within Human Cdc27 aa 662-824 / 824.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
Hela cell lysate, K562 cell lysate, human colon carcinoma tissue, human prostate tissue, human breast carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IHC-P: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 92 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]?

The catalog number for Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] is ET1612-94.

What sizes are available for Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]?

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] is available in the following size(s): 100 µL.

Is Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] in stock?

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02] used for?

Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-pro

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

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