Phospho-Cdc6 (S54) Recombinant Rabbit Monoclonal Antibody [SD08-47]

About this product

Cdc6, or cell division cycle 6, is a protein in eukaryotic cells. CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA. It is thought that the CDC6-Cdt1 complex uses ATP hydrolysis to thread DNA through the central hole of the MCM doughnut. Mutations in the binding motif of CDC6 strongly suggest that ATP binding and hydrolysis is essential for its function. The minimal requirement for DNA binding has been mapped within its 47-amino acid sequence. Furthermore, Cdc6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase, thus nuclear division is suppressed.

Specifications

Catalog number

ET1612-96

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cdc 18L antibody Cdc 6 antibody CDC18 (cell division cycle 18, S.pombe, homolog) like antibody CDC18 (S.pombe) antibody Cdc18 related protein antibody CDC18 (S.pombe) antibody Cdc18-related protein antibody Cdc18L antibody cdc6 antibody CDC6 cell division cycle 6 homolog antibody CDC6 related protein antibody CDC6-related protein antibody CDC6_HUMAN antibody Cdc6p antibody CELL CYCLE CONTROLLER CDC6 antibody Cell division control protein 6 antibody Cell division control protein 6 homolog antibody Cell division cycle 6 homolog (S. cerevisiae) antibody Cell division cycle 6 homolog antibody Cell division cycle 6, S. cerevisiae, homolog of antibody HsCDC 6 antibody HsCDC18 antibody HsCDC6 antibody p62 antibody p62 (cdc 6) antibody p62 (cdc6) antibody

Abbreviation

HsCdc18, HsCDC6, CDC6

Swiss Prot

Q99741 Human, O89033 Mouse,

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser54 of Human Cdc6 aa 41-90 / 560.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

Jurkat cell lysates, HeLa whole cell lysate, HeLa treated with 3mM Hydroxyurea for 20 hours whole cell lysate, Hela, HepG2, A431, mouse spleen tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 63 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes