TrkA+B+C Recombinant Rabbit Monoclonal Antibody [JJ084-04]

About this product

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. In neuroblastomas overexpression of TrkB correlates with unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’ tumors and this isoform may act as a dominant-negative to TrkB signaling. Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma.

Specifications

Catalog number

ET1701-16

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

BDNF/NT-3 growth factors receptor antibody gp140trk antibody GP145-TrkB antibody GP145-TrkC antibody High affinity nerve growth factor receptor antibody MTC antibody Neurotrophic tyrosine kinase receptor type 1 antibody Neurotrophic tyrosine kinase receptor type 2 antibody Neurotrophic tyrosine kinase receptor type 3 antibody NT-3 growth factor receptor antibody NTRK1 antibody NTRK2 antibody NTRK3 antibody p140-TrkA antibody TRK antibody Trk-A antibody Trk-B antibody Trk-C antibody TRK1-transforming tyrosine kinase protein antibody TRKA antibody TRKB antibody TrkB tyrosine kinase antibody TRKC antibody TrkC tyrosine kinase antibody Tropomyosin-related kinase A antibody Tropomyosin-related kinase B antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody

Abbreviation

Trk-A, NTRK1, Trk-B, NTRK2, Trk-C, NTRK3

Swiss Prot

P04629 Human, Q16288 Human, Q16620 Human, Q3UFB7 Mouse, P15209 Mouse, Q6VNS1 Mouse, P35739 Rat, Q63604 Rat, Q03351 Rat

Cellular Locus

Membrane, Endosome membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human TrkB aa 773-822 / 822.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Rat brain tissue lysate, mouse brain tissue lysate, N2A, SH-SY-5Y, mouse liver tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 92 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes