pro Caspase-9 Recombinant Rabbit Monoclonal Antibody [JJ08-05]

About this product

Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. It is an initiator caspase, critical to the apoptotic pathway found in many tissues. Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury. Without correct function, abnormal tissue development can occur leading to abnormal function, diseases and premature death. Caspase-9 loss-of-function mutations have been associated with immunodeficiency/lymphoproliferation, neural tube defects, and Li-Fraumeni-like syndrome. Increased caspase-9 activity is implicated in the progression of amyotrophic lateral sclerosis, retinal detachment, and slow-channel syndrome, as well as various other neurological, autoimmune, and cardiovascular disorders.

Specifications

Catalog number

ET1701-22

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Caspase9 APAF-3 antibody APAF3 antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase-9 subunit p10 antibody Caspase-9 antibody Caspase 9 antibody Caspase9 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody ICELAP6 antibody MCH6 antibody

Abbreviation

CASP-9, APAF-3, ICE-LAP6, CASP9

Swiss Prot

P55211 Human

Cellular Locus

Cytosol,Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Caspase-9 aa 20-69 / 416.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, HeLa treated with 3μM staurosporine for 4 hours cell lysate, human placenta tissue, human skin tissue, human cervix uteri tissue, Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes