AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

About this product

Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Specifications

Catalog number

ET1701-26

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

5730550L01Rik antibody Ago 3 antibody Ago3 antibody AGO3_HUMAN antibody argonaute 3 antibody Argonaute3 antibody eIF 2C 3 antibody eIF-2C 3 antibody eIF2C 3 antibody Eif2c3 antibody EIF2C3 protein antibody Eukaryotic translation initiation factor 2C 3 antibody Eukaryotic translation initiation factor 2C3 antibody FLJ12765 antibody hAgo3 antibody MGC86946 antibody Protein argonaute-3 antibody

Abbreviation

Argonaute3, hAgo3, eIF-2C 3, eIF2C 3, AGO3

Swiss Prot

Q9H9G7 Human, Q8CJF9 Mouse

Cellular Locus

Cytoplasm,Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human EIF2C3/ Argonaute 3 aa 689-860/860.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HeLa cell lysate, C6 cell lysate, N2A, SHG-44, F9, NCCIT, rat spinal cord tissue, rat brain tissue, mouse brain tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 97 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes