Phospho-Raf1 (S621) Recombinant Rabbit Monoclonal Antibody [JJ085-05]

About this product

Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.

Specifications

Catalog number

ET1701-3

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

c Raf antibody C-raf antibody C-Raf proto-oncogene, serine/threonine kinase antibody CMD1NN antibody Craf 1 transforming gene antibody cRaf antibody Craf1 transforming gene antibody EC 2.7.11.1 antibody kinase Raf1 antibody Murine sarcoma 3611 oncogene 1 antibody NS5 antibody Oncogene MIL antibody Oncogene RAF1 antibody OTTHUMP00000160218 antibody OTTHUMP00000207813 antibody OTTHUMP00000209389 antibody Protein kinase raf 1 antibody Proto-oncogene c-RAF antibody Raf 1 antibody Raf 1 proto oncogene serine/threonine kinase antibody RAF antibody Raf proto oncogene serine/threonine protein kinase antibody RAF proto-oncogene serine/threonine-protein kinase antibody RAF-1 antibody RAF1 antibody RAF1_HUMAN antibody Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody v raf 1 murine leukemia viral oncogene homolog 1 antibody v-raf murine sarcoma viral oncogene homolog 1 antibody v-raf-1 murine leukemia viral oncogene-like protein 1 antibody vraf1 murine leukemia viral oncogene homolog 1 antibody

Abbreviation

cRaf, RAF1

Swiss Prot

P04049 Human, Q99N57 Mouse, P11345 Rat

Cellular Locus

Cytoplasm, Cell membrane, Mitochondrion, Nucleus.

Host

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser621 of Human Raf1 aa 591-640 / 648.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Human skeletal muscle tissue lysates, rat trachea tissue, human muscle tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:100-1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 73 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes