Phospho-PLK1 (T210) Recombinant Rabbit Monoclonal Antibody [JJ20-58]

About this product

Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. During interphase, PLK1 localizes to centrosomes. In early mitosis, it associates with mitotic spindle poles. A recombinant GFP-PLK1 protein localizes to centromere/kinetochore region, suggesting a possible role for chromosome separation. Plk1 is an early trigger for G2/M transition. Plk1 supports the functional maturation of the centrosome in late G2/early prophase and establishment of the bipolar spindle. Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Plk phosphorylates and activates components of the anaphase-promoting complex (APC).

Specifications

Catalog number

ET1701-33

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cell cycle regulated protein kinase antibody PLK 1 antibody PLK antibody PLK-1 antibody plk1 antibody PLK1_HUMAN antibody Polo like kinase 1 antibody Polo-like kinase 1 antibody Serine/threonine protein kinase 13 antibody Serine/threonine protein kinase PLK1 antibody Serine/threonine-protein kinase 13 antibody Serine/threonine-protein kinase PLK1 antibody STPK 13 antibody STPK13 antibody

Abbreviation

PLK-1, STPK13, PLK1

Swiss Prot

P53350 Human, Q07832 Mouse

Cellular Locus

Nucleus, Chromosome, Cytoplasm, Midbody.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr210 of Human PLK1 aa 181-230 / 603.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, FC, IHC-P

Positive Control

Mouse thymus tissue, MCF-7.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:10-1:50 ;IF-Tissue: 1:10-1:50 ;FC: 1:10-1:50 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes