Phospho-AMPK alpha 2 (S345) Recombinant Rabbit Monoclonal Antibody [JJ08-19]

About this product

Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein.

Specifications

Catalog number

ET1701-37

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody AAPK2_HUMAN antibody ACACA kinase antibody Acetyl-CoA carboxylase kinase antibody AMPK alpha 2 chain antibody AMPK subunit alpha-2 antibody AMPK2 antibody AMPKa2 antibody AMPKalpha2 antibody HMGCR kinase antibody Hydroxymethylglutaryl-CoA reductase kinase antibody PRKAA antibody PRKAA2 antibody Protein kinase AMP activated alpha 2 catalytic subunit antibody Protein kinase AMP activated catalytic subunit alpha 2 antibody

Abbreviation

AMPK subunit alpha-2, ACACA kinase, HMGCR kinase, PRKAA2

Swiss Prot

P54646 Human, Q8BRK8 Mouse, Q09137 Rat

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser345 of Human AMPK alpha 2 aa 338-352 / 552.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P, FC

Positive Control

HEK-293 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, HEK-293, human skeletal muscle tissue, rat skeletal muscle tissue, rat heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 62 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes