14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ084-3]

About this product

The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.

Specifications

Catalog number

ET1701-4

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

14-3-3 protein beta/alpha Protein 1054 Protein kinase C inhibitor protein 1 KCIP-1 14-3-3 protein beta/alpha, N-terminally processed YWHAB 14-3-3 protein zeta/delta YWHAZ

Abbreviation

KCIP-1, YWHAB, YWHAZ

Swiss Prot

P31946 Human, P63104 Human, P63101 Mouse, Q9CQV8 Mouse, P35213 Rat, P63102 Rat

Cellular Locus

Cytoplasm, Melanosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human 14-3-3 aa 1-122 / 246.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

NIH/3T3 cell lysate, SK-Br-3 cell lysate, human skin tissue lysates.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 28 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes