JNK1+JNK3 Recombinant Rabbit Monoclonal Antibody [JJ08-86]

About this product

c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).

Specifications

Catalog number

ET1701-47

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

c Jun N terminal kinase 3 antibody c Jun N-terminal kinase 1 antibody JNK 46 antibody JNK antibody JNK1 antibody JNK1A2 antibody JNK21B1/2 antibody JNK3 alpha protein kinase antibody JNK3 antibody JNK3A antibody JUN N terminal kinase antibody MAP kinase 10 antibody MAP kinase 8 antibody MAP kinase p49 3F12 antibody MAPK 10 antibody MAPK10 antibody MAPK8 antibody Mitogen activated protein kinase 10 antibody Mitogen activated protein kinase 8 antibody Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody p493F12 antibody p54bSAPK antibody PRKM10 antibody PRKM8 antibody SAPK1 antibody SAPK1b antibody SAPK1c antibody Stress activated protein kinase 1 antibody Stress activated protein kinase 1b antibody Stress activated protein kinase 1c antibody Stress activated protein kinase beta antibody Stress activated protein kinase JNK3 antibody

Abbreviation

MAP kinase 8, MAPK 8, SAPK1c, MAPK8, MAP kinase 10, MAPK 10, SAPK1b, MAPK10

Swiss Prot

P45983 Human, P53779 Human, Q61831 Mouse, Q91Y86 Mouse, P49185 Rat, P49187 Rat

Cellular Locus

Cytoplasm, Nucleus, Membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human JNK1 + JNK3 aa 1-423 / 427.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, IHC-P

Positive Control

NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 48/53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes