MGMT Recombinant Rabbit Monoclonal Antibody [JJ089-6]

About this product

MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

Specifications

Catalog number

ET1701-55

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

6 O methylguanine DNA methyltransferase antibody 6-O-methylguanine-DNA methyltransferase antibody Agat antibody AGT antibody AI267024 antibody EC 2.1.1.63 antibody Methylated DNA protein cysteine methyltransferase antibody Methylated-DNA--protein-cysteine methyltransferase antibody Methylguanine DNA methyltransferase antibody MGC107020 antibody MGMT antibody MGMT_HUMAN antibody O 6 methylguanine DNA alkyltransferase antibody O 6 methylguanine DNA methyltransferase antibody O-6-methylguanine-DNA methyltransferase antibody O-6-methylguanine-DNA-alkyltransferase antibody

Abbreviation

MGMT

Swiss Prot

P16455 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human MGMT aa 1-140 / 207.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

MCF7 cell lysate, Jurkat cell lysate, HCT 116 cell lysate, HeLa cell lysate, MCF7, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes