CD3D Recombinant Rabbit Monoclonal Antibody [JJ08-97]

About this product

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ΩΩ) or a heterodimer of zeta and eta chains (Ωh). The Ω and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the Ω chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the Ω chain ITAMs has been solved.

Specifications

Catalog number

ET1701-57

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD3 antigen delta subunit antibody CD3 delta antibody CD3d antibody CD3d antigen delta polypeptide (TiT3 complex) antibody CD3d molecule delta (CD3-TCR complex) antibody CD3D_HUMAN antibody IMD19 antibody OKT3 delta chain antibody T cell receptor T3 delta chain antibody T-cell receptor T3 delta chain antibody T-cell surface glycoprotein CD3 delta chain antibody T3D antibody

Abbreviation

CD3D

Swiss Prot

P04234 Human

Cellular Locus

Membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human CD3D aa 60-171 / 171.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IP

Positive Control

Jurkat cell lysates, Jurkat, human spleen tissue, human thymus tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 19 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes