SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]

About this product

The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis. The mammalian PcG proteins are regulatory proteins important for Hox gene expression, axial skeleton development and the control of proliferation and survival of hematopoietic cells. By inducing changes in chromatin structure, the PcG proteins are part of a cellular memory system that is responsible for gene activity being inherited to progeny cells. PcG proteins silence gene expression through the formation of multimeric protein complexes with different compositions. Manipulating the expression-levels of various PcG proteins in mammalian cell lines results in cellular transformation, which may be a link between the chromatin-associated PcG proteins and cancer. Polycomb protein SUZ12, also designated ChET 9 protein or joined-to-JAZF1 protein, is a nuclear protein belonging to the VEFS (VRN2-EMF2-FIS2-SUZ12) family. SUZ12 has been detected at the breakpoints of a certain recurrent chromosomal translocation which has been reported in endometrial stromal sarcoma. It is a component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7.

Specifications

Catalog number

ET1701-62

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ChET 9 protein antibody CHET9 antibody Chromatin precipitated E2F target 9 protein antibody JJAZ1 antibody Joined to JAZF1 protein antibody KIAA0160 antibody Polycomb protein SUZ12 antibody Suppressor of zeste 12 homolog antibody Suppressor of zeste 12 protein homolog antibody SUZ12 antibody SUZ12 polycomb repressive complex 2 subunit antibody SUZ12_HUMAN antibody

Abbreviation

ChET 9 protein, SUZ12

Swiss Prot

Q15022 Human, Q80U70 Mouse,

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human SUZ12 aa 1-150/739.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IP

Positive Control

MCF-7 cells lysates, NIH/3T3 cell lysate, PC-12 cell lysate, Raw264.7 cell lysate, SW480, MCF-7, Hela, SKOV-3.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 83 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes