Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] — image 1 of 3

Product details

Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07]

Catalog number ET1701-64

Price for ET1701-64

Size / count 100 µL

About this product

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

Specifications

Catalog number: ET1701-64
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody
Abbreviation: Hist1h3a, Hist1h3b
Swiss Prot: P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, P84228 Mouse, Q6LED0 Rat
Cellular Locus: Nucleus, Chromosome.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within human Histone H3 aa 85-136/136.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, ChIP, IP
Positive Control: HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HEK-293 cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, HeLa, human kidney tissue, human skin tissue, mouse liver tissue, mouse kidney tissue, rat liver tissue, rat kidney tissue, rat skin tissue, human liver tissue, mouse testis tissue, rat testis tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:20,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:100 ;IHC-P: 1:5,000-1:10,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;IP: 1-2μg/sample
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 15 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07]?

The catalog number for Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] is ET1701-64.

What sizes are available for Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07]?

Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] is available in the following size(s): 100 µL.

Is Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] in stock?

Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.