E2F1 Recombinant Rabbit Monoclonal Antibody [JJ092-02]

About this product

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

Specifications

Catalog number

ET1701-73

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DmelCG6376 antibody Dmel_CG6376 antibody drosE2F1 antibody E (Sev-CycE)3A antibody E (var)3-93E antibody E2-promoter binding facto antibody E2F 1 antibody E2F transcription factor 1 antibody E2F-1 antibody E2f-PA antibody E2f-PB antibody E2f-PC antibody E2F1 antibody E2f1 E2F transcription factor 1 antibody E2F1_HUMAN antibody Evar (3)164 antibody KIAA4009 antibody l (3)07172 antibody l (3)j3B1 antibody l (3)j3C2 antibody l (3)rM729 antibody mKIAA4009 antibody OTTHUMP00000030661 antibody PBR3 antibody PRB binding protein E2F 1 antibody PRB-binding protein E2F-1 antibody RBAP 1 antibody RBAP-1 antibody RBAP1 antibody RBBP-3 antibody RBBP3 antibody RBP 3 antibody RBP3 antibody Retinoblastoma-associated protein 1 antibody Retinoblastoma-binding protein 3 antibody Transcription factor E2F1 antibody

Abbreviation

E2F-1, RBAP-1, RBBP-3

Swiss Prot

Q01094 Human, Q61501 Mouse, O09139 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human E2F1 aa 371-404 / 437.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

HT-29 cell lysate, SW480 cell lysate, SW620 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, rat heart tissue lysate, Hela, MCF-7, HepG2, human tonsil tissue, mouse pancreas tissue, rat heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:10,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:1,000-1:5,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 47 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes